Plasmid_Backbone

Part:BBa_K106672

Designed by: Andrew Horwitz   Group: iGEM08_UCSF   (2008-10-28)


AarI AD acceptor vector (pRS305, Gal1P, Adh1t)

This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Gal1 promoter (Gal1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal EcoRI site found at 5818
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal EcoRI site found at 5818
    Illegal NotI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal EcoRI site found at 5818
    Illegal BamHI site found at 5
    Illegal XhoI site found at 924
    Illegal XhoI site found at 6629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal EcoRI site found at 5818
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2218
    Illegal EcoRI site found at 5818
    Illegal NgoMIV site found at 535
    Illegal AgeI site found at 1831
    Illegal AgeI site found at 6242
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4254
    Illegal SapI.rc site found at 5336


[edit]
Categories
//chassis/eukaryote/yeast
//plasmidbackbone/operation
Parameters
None